Coding

Part:BBa_K1965044:Design

Designed by: Katja Leben   Group: iGEM16_Slovenia   (2016-10-17)


CIBN:cPPV


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 556
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 142
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 592
    Illegal BsaI.rc site found at 772


Design Notes

This sequence is inserted into pcDNA3 vector appropriate for expression in mammal cells.


Source

CIBN - Arabidopsis thaliana
cPPV - Plum pox virus

References